Top 100 Research Topics & Titles about Food & TVL When you look for a good research paper topic, you can easily become the severest critic of any proposed idea. It follows a strict process of data collection. In parallel, DNA from sample G147/08 was also extracted and purified according to a modified CTAB method (see Appendix S1) [21]. MON810 content measured by ddPCR in five series of seven target concentrations. Fast food vs. slow food: what will be the trend in the future? To be employed in routine testing, methods based on ddPCR shall be properly validated through ring-trials and verified during their introduction in laboratories to demonstrate their fitness for the purpose. Several advantages are proposed for the use of dPCR instead of qPCR in routine GMO testing: 1) it enables absolute target copy number to be detected and avoids the amplification efficiency bias observed with qPCR [11], [19], 2) it overcomes dependence on the availability of references or standards [18], 3) it provides data with the high precision and confidence necessary for metrological use [11], [19], 4) it provides more accurate data at low target copy numbers than qPCR [20], allowing quantification of low GMO content, and 5), because of its tolerance to inhibitors as an end-point measurement, it can reduce the biases linked to matrix type often observed with qPCR [18]. During qPCR tests, we detected the presence of inhibition in the stock DNA solution of two samples (wheat seed-powder flour with maize contamination and maize feed), as indicated by the large differences in MON810 content (cv>25%) calculated from different sample dilutions. In order to avoid biases as much as possible when comparing qPCR and ddPCR quantification, we transferred the inter-laboratory validated qPCR hmg- and MON810-specific assays to ddPCR technology with the minimum of adaptation. Similarly, the ddPCR response for MON810 was, on average, linear from 6 to 4,340 MON810 copies (R2 = 0.9993; 0.03% to 17.9% positive droplets in average) (Figure 1). Italian culinary world: the art of matching wine to the meals. All samples used for determining the dynamic range came from serial dilution of a single stock MON810 maize DNA sample. Interestingly, for the DNA extracts obtained with NSF, very low differences were observed between the MON810 content determined by ddPCR in the stock (presenting inhibition with qPCR) and the diluted DNA solutions for both matrices (Table S8 in Appendix S2). To conduct quantitative research, methods like online survey, questionnaires, sampling methods etc. How do different types of wood for smoking affect the taste of meat? Quantitative research involves methodical analysis of phenomena by means of collecting quantifiable data and then carrying out mathematical, statistical, or computing techniques. This work was co-financed by the Slovenian Ministry of Economic Development and Technology, Metrology Institute (MIRS) in the frame of the contract no. CiteScore values are based on citation counts in a range of four years (e.g. The absolute limit of detection (aLOD) is the lowest target copy number in a sample that can be reliably detected, but not necessarily quantified [25]. Garlic and onions: Ayurveda point of view. Wrote the paper: DM MM KG JZ. 4 - All-natural claims: Are they worth it? Quantitative research is most common encountered as part of formal or conclusive research, but is also sometimes used when conducting exploratory research.Quantitative research techniques are part of primary research.. Quantitative research differs from qualitative research in the following ways:. The impact of the size of the plate on our level of hunger. https://doi.org/10.1371/journal.pone.0062583.s010, https://doi.org/10.1371/journal.pone.0062583.s011. Low-glycemic index diet to treat diseases. One of the limitations is the need to choose target sequences with similar, short lengths. Acceptance criterion for trueness is that the measured value has to be within ±25% of the accepted reference value over the whole dynamic range [24], [25]. Fast Food Research Paper Topics for Fast Food Lovers and Haters Fast food is firmly established in our lives, and not everyone can resist the temptation to taste it. Comparison of duplex ddPCR quantification on digested and non-digested genomic DNA. If you continue, Enzymatic digestion of MON810 genomic DNA (gDNA) with TaqI (New England Biolabs GmbH, Frankfurt am Main, Germany) was performed as described (see Appendix S1) [19]. Vegetarianism: a new trend or philosophy? DNA was extracted and purified from 200 mg of starting material for all samples using the NucleoSpin Food kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Duplex ddPCR reaction mixes were prepared as follows. Eating disorders and beauty standards: a quantitative evaluation. In the case of MON810 maize, results of the two dimensional analyses of the droplet signals in digested and undigested DNA samples (Figure S1 in Supporting Information) suggests the absence of linkage between MON810 positive droplets and hmg positive droplets, thus confirming the independence of the two targets. The most harmful preservatives in fast food. Table S8. The same primers and probes were used for both qPCR and ddPCR experiments (see Appendix S1 and Table S2). The average value of the pooled ddPCR duplex assay data at each dilution level of the dynamic range showed good agreement with the reference value of 3.85% cp/cp (ERM-BF413gk) (Table 1). Set-up and number of reactions needed for simultaneous quantification of 23 samples with qPCR and ddPCR. Additionally, the repeatability of the duplex assay in measurements of the MON810 content appears slightly better that of the singleplex assays. If more samples must be handled simultaneously, ddPCR shows even better throughput and smaller cost than qPCR (see Tables S11 and S12 in Appendix S2). Therefore, any of the topics mentioned above would fit perfectly for the task of picking research topics on the food industry. The dilution series consisted of 14 solutions containing from approximately 118000 to 0.02 copies of hmg, and from 4300 to 0.0008 copies of MON810 per 20 µL, respectively). 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